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• 2-Deoxy derivative is a partial agonist of the intracellular messenger inositol 3,4,5,6-tetrakisphosphate in the epithelial cell line T84.
• A FlAsH-based cross-linker to study protein interactions in living cells.
• A dual parameter FRET probe for measuring PKC and PKA activity in living cells.
• A membrane-permeant, bioactivatable derivative of Ins(1,3,4)P3 and its effect on Cl(-)-secretion from T84 cells.
• A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins.
• A new player in the game: epithelial cathepsin S in early cystic fibrosis lung disease.
• A phosphoinositide conversion mechanism for exit from endosomes
• A rapidly reversible chemical dimerizer system to study lipid signaling in living cells.
• A single-cell model of PIP<inf>3</inf> dynamics using chemical dimerization
• A small-molecule FRET probe to monitor phospholipase A2 activity in cells and organisms.
• Activation of membrane-permeant caged PtdIns(3)P induces endosomal fusion in cells.
• Airway mucus obstruction triggers macrophage activation and matrix metalloproteinase 12-dependent emphysema.
• Airway surface liquid volume regulation determines different airway phenotypes in liddle compared with betaENaC-overexpressing mice.
• Amino acids for Diels-Alder reactions in living cells.
• Analysis of protein complex hierarchy in living cells.
• Annexin A4 self-association modulates general membrane protein mobility in living cells.
• Antagonists of myo-inositol 3,4,5,6-tetrakisphosphate allow repeated epithelial chloride secretion.
• Bifunctional Sphingosine for Cell-Based Analysis of Protein-Sphingolipid Interactions
• Bioactivatable derivatives of 8-substituted cAMP-analogues
• Bioorganic Chemistry Meeting in Wales
• CFTR regulates early pathogenesis of chronic obstructive lung disease in βENaC-overexpressing mice.
• Caged lipids as tools for investigating cellular signaling.
• Calcium-dependent regulation of NF-(kappa)B activation in cystic fibrosis airway epithelial cells.
• Can we see PIP(3) and hydrogen peroxide with a single probe?
• Cellular delivery and photochemical release of a caged inositol-pyrophosphate induces PH-domain translocation in cellulo
• Cellular uptake of PNA--terpyridine conjugates and its enhancement by Zn2+ ions.
• Challenges in studying phospholipid signaling.
• Chapter 6 Small molecule-based FRET probes
• Chemical activators of protein phosphatase-1 induce calcium release inside intact cells.
• Chemical biologists gather in Heidelberg.
• Climbing to the next step: photosensitive protein labels.
• Closing the gap between chemistry and biology.
• Conformational analysis of a genetically encoded FRET biosensor by SAXS.
• Contribution of fluorophores to protein kinase C FRET probe performance.
• Controlling protein function by caged compounds
• Covalent labeling of biomolecules in living cells
• Cross-talk between calcium and cAMP-dependent intracellular signaling pathways. Implications for synergistic secretion in T84 colonic epithelial cells and rat pancreatic acinar cells.
• D-myo-Inositol 1,4,5,6-tetrakisphosphate produced in human intestinal epithelial cells in response to Salmonella invasion inhibits phosphoinositide 3-kinase signaling pathways.
• DOTAM Derivatives as Active Cartilage-Targeting Drug Carriers for the Treatment of Osteoarthritis
• Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites
• Development of Fluorescent Probes for Small Molecules
• Does cellular hydrogen peroxide diffuse or act locally?
• Exclusive photorelease of signalling lipids at the plasma membrane
• FRET probes to monitor phospholipase A2 activity.
• FRET-based and other fluorescent proteinase probes.
• FluoQ: a tool for rapid analysis of multiparameter fluorescence imaging data applied to oscillatory events.
• Fluorescence and bioluminescence procedures for functional proteomics.
• Fluorescent revelations.
• Genetic code expansion for multiprotein complex engineering
• Genetic encoding of a bicyclo[6.1.0]nonyne-charged amino acid enables fast cellular protein imaging by metal-free ligation.
• Genetically encoded FRET probe for PKC activity based on pleckstrin.
• Genetically encoded copper-free click chemistry.
• Genetically encoded fluorescent indicator for imaging NAD(+)/NADH ratio changes in different cellular compartments.
• Heterogeneity and timing of translocation and membrane-mediated assembly of different annexins.
• Highly Stable trans-Cyclooctene Amino Acids for Live-Cell Labeling
• HyPer-3: a genetically encoded H(2)O(2) probe with improved performance for ratiometric and fluorescence lifetime imaging.
• Imaging H2O2 microdomains in receptor tyrosine kinases signaling.
• Imaging lipids in living cells.
• Imaging probes--Introduction.
• In vivo imaging of mouse tumors by a lipidated cathepsin S substrate.
• In vivo profiling and visualization of cellular protein-lipid interactions using bifunctional fatty acids.
• In vivo visualization of osteoarthritic hypertrophic lesions
• Inositol 1,3,4-trisphosphate acts in vivo as a specific regulator of cellular signaling by inositol 3,4,5,6-tetrakisphosphate.
• Inositol pentakisphosphate isomers bind PH domains with varying specificity and inhibit phosphoinositide interactions.
• Inositol polyphosphate derivative inhibits Na+ transport and improves fluid dynamics in cystic fibrosis airway epithelia.
• Intracellular sphingosine releases calcium from lysosomes
• Investigation of the ligand spectrum of human sterol carrier protein 2 using a direct mass spectrometry assay.
• Labeling lipids for imaging in fixed cells
• Labeling lipids for imaging in live cells
• Lack of neutrophil elastase reduces inflammation, mucus hypersecretion, and emphysema, but not mucus obstruction, in mice with cystic fibrosis-like lung disease.
• Lipid Discovery by Combinatorial Screening and Untargeted LC-MS/MS
• Lipid-induced phenotypes.
• Live-cell STED microscopy with genetically encoded biosensor
• Live-cell imaging of enzyme-substrate interaction reveals spatial regulation of PTP1B.
• Long-term uncoupling of chloride secretion from intracellular calcium levels by Ins(3,4,5,6)P4.
• Manipulating cell signaling with subcellular spatial resolution
• Membrane lipids tune synaptic transmission by direct modulation of presynaptic potassium channels.
• Membrane lipids tune synaptic transmission by direct modulation of presynaptic potassium channels. [(Neuron 81, 19, (2014) 787-799]
• Membrane-Permeant 3-OH-Phosphorylated Phosphoinositide Derivatives.
• Membrane-bound FRET probe visualizes MMP12 activity in pulmonary inflammation.
• Membrane-permeant esters of inositol polyphosphates, chemical syntheses and biological applications
• Membrane-permeant phosphoinositide derivatives as modulators of growth factor signaling and neurite outgrowth.
• Membrane-permeant, bioactivatable derivatives of inositol polyphosphates and phosphoinositides
• Minimal tags for rapid dual-color live-cell labeling and super-resolution microscopy.
• Molecular tools for cell and systems biology.
• Multiparameter imaging for the analysis of intracellular signaling.
• Optotaxis: Caged Lysophosphatidic Acid Enables Optical Control of a Chemotactic Gradient
• PIP₃ induces the recycling of receptor tyrosine kinases.
• PLCβ isoforms differ in their subcellular location and their CT-domain dependent interaction with Gαq.
• Photoactivatable and cell-membrane-permeable phosphatidylinositol 3,4,5-trisphosphate.
• Photoswitchable diacylglycerols enable optical control of protein kinase C
• Plasma membrane phosphoinositide balance regulates cell shape during Drosophila embryo morphogenesis.
• Principles for designing fluorescent sensors and reporters.
• Probing lipid- and drug-binding domains with fluorescent dyes.
• Probing phospholipase a(2) with fluorescent phospholipid substrates.
• Prodrugs of biologically active phosphate esters.
• Protein tango: the toolbox to capture interacting partners.
• Protein translocation as a tool: The current rapamycin story.
• Rapid development of genetically encoded FRET reporters.
• Reporters to monitor cellular MMP12 activity
• Reversible chemical dimerizer-induced recovery of PIP2 levels moves clathrin to the plasma membrane
• Role of guanylyl cyclase and cGMP-dependent protein kinase in long-term potentiation.
• Selective fluorescence labeling of lipids in living cells.
• Simultaneous protein tagging in two colors.
• Simultaneous recording of multiple cellular events by FRET.
• Single- and dual-parameter FRET kinase probes based on pleckstrin.
• Spatially resolved monitoring of neutrophil elastase activity with ratiometric fluorescent reporters.
• Spatiotemporal control of endocytosis by phosphatidylinositol-3,4-bisphosphate.
• Sphingosine-1-phosphate lyase deficient cells as a tool to study protein lipid interactions
• Switching heterotrimeric G protein subunits with a chemical dimerizer.
• Synthesis of DL-myo-inositol 1-phosphate and its thiophosphate analogue
• Synthesis of caged myo-inositol 1,3,4,5-tetrakisphosphate
• Synthesis of thiophosphate analogues of DL-myo-inositol 1.2-Cyclic phosphate
• T-CrAsH: a heterologous chemical crosslinker.
• Target-activated prodrugs (TAPs) for the autoregulated inhibition of MMP12
• The ENaC-overexpressing mouse as a model of cystic fibrosis lung disease.
• The European Molecular Biology Laboratory (EMBL)--its anniversary and its chemistry.
• The chemical biology of phosphoinositide 3-kinases.
• The fatty acid composition of diacylglycerols determines local signaling patterns.
• The power of fluorogenic probes.
• Tissue clearing for optical anatomy.
• Transfection of cells with DNA encoding a visible fluorescent protein-tagged lipid-binding domain
• Versatile reagents to introduce caged phosphates
• Vicinal thiols are involved in inositol 1,2,3,5,6-pentakisphosphate 5-phosphatase activity from fetal calf thymus.
• Visualization of intracellular hydrogen peroxide with HyPer, a genetically encoded fluorescent probe.
• cis,cis-cyclohexane 1,3,5-triol polyphosphates release calcium from Neurospora crassa via an unspecific Ins 1,4,5-P3 receptor.
• mCLCA3 does not contribute to calcium-activated chloride conductance in murine airways.
• myo-Inositol 3,4,5,6-tetrakisphosphate inhibits an apical calcium-activated chloride conductance in polarized monolayers of a cystic fibrosis cell line